Invitrogen™ CD307d (FcRL4) Monoclonal Antibody (413D12), PerCP-eFluor™ 710, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-113-0800	Invitrogen™ 46307942	100 Tests

Description

Description: The 413D12 monoclonal antibody reacts with human CD307d, which is also known as FcRL4, Fc receptor-like 4, FcRH4, IRTA4, or IGFP2. CD307d is an inhibitory receptor that is highly expressed on a subset of memory B cells resident in epithelial tissues and marginal zones of mucosal lymphoid tissues such as tonsils. CD307d has been shown to recruit the phosphatase SHP-1, thereby acting as an inhibitory receptor in B cells. CD307d is homologous to FcgammaRI and specifically binds IgA. To date, CD307d is the only known inhibitory receptor for IgA. Expression of CD307d is increased on memory B cells from patients with chronic infections such as HIV, suggesting that enhanced expression of inhibitory receptors, such as CD307d, may contribute to the inefficiency of antibody responses against HIV. Applications Reported: This 413D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 413D12 antibody has been pre-titrated and tested by flow cytometric analysis of human tonsil tissue or transfected 293T cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however...

This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains three immune-receptor tyrosine-based inhibitory motifs. This protein may play a role in the function of memory B-cells in the epithelia. Aberrations in the chromosomal region encoding this gene are associated with non-Hodgkin lymphoma and multiple myeloma.

Specifications

• Antigen: CD307d (FcRL4)
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: PerCP-eFluor 710
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q96PJ5
• Gene ID (Entrez): 83417
• Primary or Secondary: Primary
• Purification Method: Affinity Chromatography

• Clone: 413D12
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2b κ
• Target Species: Human
• Gene: FCRL4
• Gene Alias: CD307d; fc receptor homolog 4; Fc receptor like 4; Fc receptor-like 4; Fc receptor-like protein 4; FcRH4; FCRL4; FcR-like protein 4; hIFGP2; IFGP family protein 2; IFGP2; IGFP2; immune receptor translocation-associated protein 1; immunoglobulin superfamily Fc receptor, gp42; immunoglobulin superfamily receptor translocation associated 1; IRTA1; MGC150522; MGC150523
• Gene Symbols: FCRL4
• Product Type: Antibody