Invitrogen™ CD31 (PECAM-1) Monoclonal Antibody (Gi18), Biotin, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ BMS137BT

Catalog No. 50-177-91

Description

Description: BMS137 is suitable as primary antibody in staining for FACS analysis. BMS137FI can be used for direct staining of cells. BMS137BT can be used in a two-step procedure using the biotin-(strept)-avidin system. BMS137 is also suitable for Western blot analysis using non-reducing conditions. The antibody can be used in immunohistochemistry using cryostat sections or paraffin-embedded tissue. BMS137 is suitable for ELISA application. PECAM-1 is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation. PECAM-1 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis. It has also been found on platelets, monocytes, neutrophils and CD8+ T cells. Bone marrow stem cells and transformed cell lines of the myeloid and megakaryocytic lineage also express PECAM-1. Interestingly, PECAM-1 was also detected on human, mouse and rat solid tumor lines. Recent studies suggest a role for PECAM-1 in the inflammatory process and leukocyte-endothelial interaction. PECAM-1 is an early and sensitive marker for tumor-induced angiogenesis. Several data have suggested that PECAM-1 may be involved in the process of angiogenesis in a developing vertebrate embryo as well as during metastases formation. Applications Tested: Flow Cytometry. Purity: >95%.

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

Specifications

• Antigen: CD31 (PECAM-1)
• Classification: Monoclonal
• Concentration: 10 μL/Test
• Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.2-7.4
• Gene Accession No.: P16284
• Gene Symbols: Pecam1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5175
• Content And Storage: 2°C to 8°C
• Form: Liquid

• Applications: Flow Cytometry
• Clone: Gi18
• Conjugate: Biotin
• Gene: Pecam1
• Gene Alias: C85791; CD31; CD31 antigen; CD31, PECAM-1; CD31/EndoCAM; endoCAM; GPIIA'; I79_008304; PECA1; Pecam; PECAM1; Pecam-1; platelet and endothelial cell adhesion molecule 1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1; type I transmembrane endothelial adhesion molecule
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1