CD314 (NKG2D) Monoclonal Antibody (CX5), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES 16588285

Catalog No. 50-145-99

Description

Description: The CX5 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1alpha, -beta, -gamma, -delta, -ε and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1^+ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells. Applications Reported: The CX5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of binding of NKG2D to its ligands, RAE-1 and H60, and inhibition NKG2D-dependent NK cell-mediated cytotoxicity against NKG2D ligand-bearing tumors in vitro and in vivo . Applications Tested: The CX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that when using this antibody for staining the secondary must be Biotin anti-rat (cat 13-4813). Other secondaries may give suboptimal staining.

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.

Specifications

• Antigen: CD314 (NKG2D)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: O54709
• Gene Symbols: Klrk1
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 27007
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay, Neutralization
• Clone: CX5
• Conjugate: Functional Grade
• Gene: Klrk1
• Gene Alias: CD314; D12S2489E; D6H12S2489E; killer cell lectin like receptor K1; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; Klrk1; natural killer cell group 2D; natural killer cell receptor; NK cell receptor D; NK lectin-like receptor; Nkg2d; NKG2-D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor; NKLLR; Nkrp2; NKR-P2; NKR-P2, ortholog of human NKG2D
• Host Species: Rat
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1 κ

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.