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CD32 Mouse anti-Human, PerCP-eFluor™ 710, Clone: 6C4 (CD32), eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$143.00 - $347.00

Specifications

Antigen CD32
Clone 6C4 (CD32)
Target Species Human
Conjugate PerCP-eFluor™ 710
Isotype IgG1, kappa
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-113-0744 Non-distribution product as customer accommodation. View Documents Invitrogen
46032941
25 Tests Each for $143.00
Add to cart
 
50-112-9142 Non-distribution product as customer accommodation. View Documents Invitrogen
46032942
100 Tests Each for $347.00
Add to cart
 
Description

Description

Description: This 6C4 monoclonal antibody reacts with human CD32 (also known as Fc gammaRII). This antibody recognizes two isoforms of the receptor, Fc gammaRIIA and Fc gammaRIIB. These 40-kDa polymorphic transmembrane glycoproteins are expressed on B cells, granulocytes, monocytes, macrophages, and platelets. Moreover, these receptors are detected on natural killer cells. CD32 enables interaction between Fc gammaRII-expressing cells and opsonized antigen or IgG-containing immune complexes. Both receptors exhibit low affinity towards IgG and play a role in inflammation and autoimmune disease. This clone has been reported to inhibit Ig binding in a rosette blocking assay. Applications Reported: This 6C4 (CD32) antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6C4 (CD32) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative.

Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
Specifications

Specifications

CD32
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
6C4 (CD32)
PerCP-eFluor™ 710
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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