Invitrogen™ CD326 (EpCAM) Monoclonal Antibody (VU-1D9), eBioscience™

Catalog No.	Quantity
50-178-40	100 μg

Catalog No. 50-178-40 Supplier Invitrogen™ Supplier No. BMS171

Description

Mouse Monoclonal Antibody

BMS171 (VU-1D9) specifically reacts with human Ep-CAM, a 40kD epithelial protein expressed on baso-lateral cell surface in many epithelial tissues (but absent from mesothelial tissues). BMS171 differentiates between mesothelioma and carcinoma, a common diagnostic problem. EpCAM-1 is a homophilic and Ca2+ independent adhesion molecule that is expressed on the baso-lateral cell surface of many epithelial, but not mesothelial tissues. It is expressed de novo in squamous cell carcinoma but is absent in the majority of healthy squamous epithelia. EpCAM expression correlates with cell proliferation, dedifferentiation, and the progression of tumorigenicity. Applications Tested: Flow Cytometry, Functional Studies (Blocking), Immunohistochemistry (Frozen, Paraffin), Immunoprecipitation, Western Blotting.

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

Specifications

• Antigen: CD326 (EpCAM)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: VU-1D9
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with no preservative
• Gene: EPCAM
• Gene Accession No.: P16422
• Gene Alias: adenocarcinoma-associated antigen; CD326; cell surface glycoprotein Trop-1; DIAR5; EGP; EGP-2; EGP314; EGP40; EPCAM; Ep-CAM; EpCAM1; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; ESA; GA733-2; gp40; hEGP314; HNPCC8; human epithelial glycoprotein-2; KS 1/4 antigen; KS1/4; KSA; Ly74; lymphocyte antigen 74; M1S2; M4S1; major gastrointestinal tumor-associated protein GA733-2; mEGP314; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; panepithelial glycoprotein 314; protein 289A; Protein D5.7A; Tacsd1; Tacstd1; TROP1; Trop-1 protein; Tumor-associated calcium signal transducer 1
• Gene Symbols: EPCAM
• Host Species: Mouse
• Immunogen: Rec. Human EpCAM (CD326).
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4072
• Target Species: Human
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ