CD33 Mouse anti-Human, BB700, Clone: WM53 (also known as WM-53), BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 742217

Catalog No. BDB742217

Description

The WM53 monoclonal antibody specifically binds to human CD33. CD33 is a 67 kDa type I transmembrane glycoprotein. It is expressed on monocytes, activated T cells, myeloid progenitors, mast cells and weakly on polymorphonuclear cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes, and hematopoietic stem cells. Reports indicate that this glycoprotein can function as a sialic acid-dependent cell adhesion molecule and this function can be modulated by endogenous sialoglycoconjugates when CD33 is expressed on the membrane.

The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes. It is a polymer-based tandem dye developed exclusively by BD Biosciences. With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter). This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5. This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.

Specifications

• Antigen: CD33
• Clone: WM53 (also known as WM-53)
• Conjugate: Brilliant Blue 700 (BB700)
• Gene Alias: Siglec-3, SIGLEC3, Sialic acid-binding Ig-like lectin 3, p67, gp67, My9
• Immunogen: Acute Myeloid Leukemia Blasts
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov