Invitrogen™ CD33 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5120758

Catalog No. PIPA5120758

Description

Positive test controls include: K-562, Mouse spleen, Mouse lung, Mouse brain, Rat lung . The target is usually found in the following locations: Cell membrane, Single-pass type I membrane protein. Immunogen sequence: YYDKNSPVHG YWFREGAIIS RDSPVATNKL DQEVQEETQG RFRLLGDPSR NNCSLSIVDA RRRDNGSYFF RMERGSTKYS YKSPQLSVHV TDLTHRPKIL IPGTLEPGHS KNLTCSVSWA CEQGTPPIFS WLSAAPTSLG PRTTHSSVLI ITPRPQDHGT NLTCQVKFAG AGVTTERTIQ LNVTYVPQNP TTGIFPGDGS GKQETRAGVV H.

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

Specifications

• Antigen: CD33
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CD33
• Gene Alias: CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12489, 690492, 945
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 2.09 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: P20138, Q63994
• Gene Symbols: CD33
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 49-259 of human SIGLEC3/CD33 (NP_0017633).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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