Invitrogen™ CD339 (Jagged 1) Monoclonal Antibody (HMJ1-29), Functional Grade, eBioscience™

Armenian Hamster Monoclonal Antibody

Supplier:  Invitrogen™ 16339185

Catalog No. 50-145-23

Description

Description: This HMJ1-29 monoclonal antibody reacts with mouse Jagged-1, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g. Notch 1-4), Jagged-1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Jagged-1 is expressed by thymic lymphoid and stromal cells, as well as macrophages and CD8+CD11c+ dendritic cells in the spleen. The HMJ1-29 antibody is reported to crossreact to rat. Applications Reported: This HMD1-5 antibody has been reported for use in functional assays. Applications Tested: This HMJ1-29 antibody has been tested by flow cytometric analysis of Jagged-1-transfected CHO cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

Specifications

• Antigen: CD339 (Jagged 1)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: Q63722, Q9QXX0
• Gene Symbols: JAG1
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16449, 29146
• Content And Storage: 4°C
• Form: Liquid

• Applications: Functional Assay
• Clone: HMJ1-29
• Conjugate: Functional Grade
• Gene: JAG1
• Gene Alias: ABE2; AGS; AHD; AWS; CD339; Gsfabe2; HJ1; Htu; JAG1; jagged 1; jagged canonical Notch ligand 1; Jagged1; JAGL1; Ozz; protein jagged-1; Ser-1; Serrate-1
• Host Species: Armenian Hamster
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG