CD34, Mouse anti-Monkey, Human, Clone: QBEnd/10, Millipore Sigma™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
CBL496I25UL	MilliporeSigma CBL496I25UL	25 μL
CBL496I100	MilliporeSigma CBL496I100UL	100 μL

Description

Anti-CD34, clone QBEnd/10, Cat. No. CBL496-I, is a mouse monoclonal antibody that detects CD34 and has been tested for use in Electron Microscopy, Flow Cytometry, Immunofluorescence and Fluorescence Activated Cell Sorting (FACS), Immunohistochemistry (Paraffin), and Western Blotting.

Hematopoietic progenitor cell antigen CD34 (UniProt: P28906; also known as CD34) is encoded by the CD34 gene (Gene ID: 947) in human. CD34 is a highly glycosylated single-pass type I membrane protein that is expressed on hematopoietic progenitor cells and small vessel endothelium of a variety of tissues. Under normal conditions, CD34+ expressing cells account for about 1 2% of the total bone marrow cells. It serves as an adhesion molecule that plays a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It is also reported to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is synthesized with a signal peptide (aa 1-31) that is cleaved off in the mature form. The mature form has an extracellular domain (aa 32-290), a transmembrane domain (aa 291-311), and a cytoplasmic domain (aa 312-385). Two isoforms of CD34 have been described that are produced by alternative splicing.

Specifications

• Antigen: CD34
• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD34 in human brain tissue sections.Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot was used to sort CD34+ cells from bone marrow. (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26). Immunofluorescence Analysis: A representative lot detected CD34 in Immunofluorescence applications (Miki, T., et. al. (2010). Mol Cancer Res. 8(5):665-76).Electron Microscopy Analysis: A representative lot detected CD34 in Electron Microscopy applications (Fina, L., et. al. (1990). Blood. 75(12):2417-26). Immunohistochemistry Analysis: A representative lot detected CD34 in Immunohistochemistry applications (Engler, J.R., et. al. (2012). PLoS One. 7(8):e43339; Fina, L., et. al. (1990). Blood. 75(12):2417-26).Flow Cytometry Analysis: A representative lot detected CD34 in Flow Cytometry applications (de Bock, C.E., et. al. (2012). Leukemia. 26(5):918-26; Fina, L., et. al. (1990). Blood. 75(12):2417-26).Western Blotting Analysis: A representative lot detected CD34 in Western Blotting applications (Fina, L., et. al. (1990). Blood. 75(12):2417-26).
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Monkey, Human
• Gene Alias: Hematopoietic progenitor cell antigen CD34
• Gene Symbols: CD34
• Isotype: IgG1 λ
• Purification Method: Protein G purified
• Test Specificity: Clone QBEnd/10 specifically detects CD34 in human and non-human primates.

• Clone: QBEnd/10
• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunomicroscopy, Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Inflammation & Immunology
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene ID (Entrez): NP_001020280
• Immunogen: Human placental endothelial membrane vesicles.
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Molecular Weight of Antigen: 40.72 kDa Calculated. This antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa.