Invitrogen™ CD34 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA578978

Catalog No. PIPA578978

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human placenta tissue, rat brain tissue, mouse brain tissue. IHC: Rat Kidney tissue, Human Lung Cancer tissue, human liver cancer tissue, human placenta tissue, mouse kidney tissue, rat kidney tissue IHC-F: Rat Placenta tissue, Rat Kidney tissue. Flow: HEL cell.

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

Specifications

• Antigen: CD34
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Cd34
• Gene Alias: (E3-independent) E2 ubiquitin-conjugating enzyme R1; AU040960; CD34; CD34 antigen; CD34 molecule; CDC34; cell division cycle 34; cell division cycle 34 homolog; cluster designation 34; cluster of differentiation antigen 34; E2 ubiquitin-conjugating enzyme R1; E2-CDC34; hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell marker CD34; Mucosialin; RP11-328D5.2; sialomucin protein; type 1 transmembrane protein; UBC3; UBCH3; UBE2R1; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 R1; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12490, 305081, 947
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.01mg sodium azide, 0.05mg thimerosal
• Gene Accession No.: P28906, Q64314
• Gene Symbols: Cd34
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD34 (366-382aa QATSRNGHSARQHVVAD).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG