Invitrogen™ CD357 (AITR/GITR) Chimeric Recombinant Rabbit Monoclonal Antibody (DTA-1)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA548161

Catalog No. PIMA548161

Description

Specificity: This antibody is specific for murine GITR, a member of the tumor necrosis factor receptor (TNFR) family that is expressed at low levels on unstimulated T cells, B cells, and macrophages. Upon activation, CD4+ and CD8+ T cells up-regulate GITR expression, whereas immunoregulatory T cells constitutively express high levels of GITR. Preparation: This antibody was raised by immunising Wistar rats with the CD25+CD4+ T cell line. Hybridoma cells were generated by fusing spleen cells from the immunized rat with P3X63Ag8.653 myeloma cells, assessed for secreting antibodies capable of neutralizing in vitro CD25+CD4+ T cell-mediated suppression of anti-CD3-stimulated T cell proliferation9, and then cloned. This DTA-1 clone secreted a monoclonal rat immunoglobulin G2a (IgG2a) that showed high neutralizing activity.

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Specifications

• Antigen: CD357 (AITR/GITR) Chimeric
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% ProClin 300
• Gene Accession No.: O35714
• Gene Symbols: Tnfrsf18
• Immunogen: This antibody was raised by immunising Wistar rats with the CD25+CD4+ T cell line.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG λ

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: DTA-1
• Conjugate: Unconjugated
• Gene: Tnfrsf18
• Gene Alias: Activation-inducible TNFR family receptor; AITR; CD357; GITR; GITR-D; glucocorticoid-induced TNFR-related protein; PRO364; TNF receptor superfamily activation-inducible protein; TNF receptor superfamily member 18; TNFRSF18; Tumor necrosis factor receptor superfamily member 18; tumor necrosis factor receptor superfamily, member 18; UNQ319; UNQ319/PRO364
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 21936
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid