CD37 Mouse anti-Human, Brilliant Violet 421, Clone: M-B371, BD Optibuild
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 742397
The M-B371 monoclonal antibody specifically recognizes CD37. CD37 is a 40-52 kDa type II integral membrane glycoprotein present on B cells from the pre-B-cell stage but not on plasma cells. CD37 is also expressed on activated, proliferating cells of germinal centers. There is low expression of CD37 on some T and myeloid cells. The function of CD37 has not yet been clearly identified.The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|GP52-40, Tetraspanin-26, TSPAN26, Tspan-26|
|Mouse BALB/c IgG1, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Brilliant Violet 421|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.|
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