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CD370 (Clec9A) Rat anti-Mouse, BUV395, Clone: 10B4 (also known as 24/04-10B4), BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 744515

Catalog No. BDB744515



The 10B4 monoclonal antibody specifically binds to mouse Clec9A. Mouse Clec9A (C-type lectin domain family member 9A) is also known as DNGR1 (Dendritic cell natural killer lectin group receptor 1). It is a type II membrane protein with a single extracellular C-type lectin domain. Clec9A is a dendritic cell subtype-restricted C-type lectin-like receptor. Clec9A is selectively expressed on plasmacytoid dendritic cells and CD8+ myeloid dendritic cells. Clec9A reportedly serves as a receptor for necrotic cells. It can mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.


CD370 (Clec9A)
Aqueous buffered solution containing ≤0.09% sodium azide.
CD370, CLC9A, Clec9a, DNGR-1, C-type lectin domain family 9 member A
Mouse Clec9A Peptide
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.
10B4 (also known as 24/04-10B4)
Brilliant Ultraviolet 395
Affinity Purified
Rat WI, also known as Wistar (outbred) IgG2a, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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