BD Horizon™ CD3e Hamster, anti-Mouse, RY586, Clone: 145-2C11, BD Horizon™

Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB568158	BD Horizon™ 568158	25 μg
BDB568157	BD Horizon™ 568157	0.1 mg

Description

The 145-2C11 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex that is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events that activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells.  In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes. Preincubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining reportedly enhances the ability of anti-CD3ε and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes.

Specifications

• Antigen: CD3e
• Applications: Flow Cytometry
• Host Species: Hamster
• Target Species: Mouse

• Clone: 145-2C11
• Conjugate: RY586
• Regulatory Status: RUO
• Primary or Secondary: Primary