Invitrogen™ CD4/CD8 Antibody Cocktail, FITC, PE

Catalog No.	Quantity
PIMA517009	100 Tests

Catalog No. PIMA517009 Supplier Invitrogen™ Supplier No. MA517009

Description

Antibody Cocktail

MA5-17009 contains a monoclonal mixture of FITC-conjugated CD4 (clone #YTS191.1, Rat IgG2b) and RPE-conjugated CD8 (clone #KT15, Rat IgG2a). This antibody is a dual-color reagent recognizing mouse CD4 and CD8 cell surface antigens. Clone YTS191.1 recognizes murine CD4, a T cell differentiation antigen expressed on thymocytes and helper/inducer T cells in the peripheral blood. Clone KT15 recognizes a non-polymorphic epitope on the murine CD8 alpha chain. Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.

CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.

Specifications

• Antigen: CD4/CD8
• Applications: Flow Cytometry
• Classification: Cocktail
• Clone: YTS191.1/KT15
• Concentration: Conc. Not Determined
• Conjugate: FITC/PE
• Formulation: PBS with 1% BSA, 5% sucrose and 0.09% sodium azide
• Gene: CD8A
• Gene Accession No.: P01731, P06332
• Gene Alias: BB154331; Cd4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; CD4 molecule; CD4 receptor; CD4mut; CD8; CD8 antigen; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8A; CD8a molecule; CD8alpha; CD8b; CD8b molecule; CD8B1; CD8beta; L3T4; LEU2; Leu-2; Leu2 T-lymphocyte antigen; Ly-2; LY3; Ly-35; Ly-4; Ly-B; Lyt2; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); LYT3; MAL; OKT8 T-cell antigen; p32; P37; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T8 T-cell antigen; T-cell antigen Leu2; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-lymphocyte differentiation antigen T8/Leu-2
• Gene Symbols: CD4, CD8A
• Host Species: Rat
• Immunogen: Clone YTS191.1: mouse thymocytes. Clone KT15: V beta 11 + T-cell clone.
• Purification Method: Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12504, 12525
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2a, IgG2b