Invitrogen™ CD4/CD8 Antibody Cocktail, FITC, PE

Catalog No.	Quantity
PIMA516802	50 Tests

Catalog No. PIMA516802 Supplier Invitrogen™ Supplier No. MA516802

Description

Antibody Cocktail

MA5-16802 is suitable for use a negative control for measurement of non-specific binding of mouse monoclonals of isotype IgG1 in rat tissue in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes. This antibody contains a monoclonal mixture of FITC-conjugated Mouse IgG1 RPE-conjugated Mouse IgG1. Reconstitute with 0.5 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti Human CD8:FITC/CD4:RPE Dual Color reeagent may be used to simultaneously to identify CD4 and CD8 positive cells. CD4, clone LT8 conjugated to FITC, is purified by ion exchange chromatography, and CD8, clone RPA-T4 conjugated to RPE, is prepared by affinity chromatography on Protein G and then purified by gel-filtration to separate the unconjugated material.

CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.

Specifications

• Antigen: CD4/CD8
• Applications: Flow Cytometry
• Classification: Cocktail
• Clone: LT8/RPA-T4
• Concentration: Conc. Not Determined
• Conjugate: FITC/PE
• Formulation: PBS with 1% BSA, 5% sucrose and 0.09% sodium azide
• Gene: CD4
• Gene Accession No.: P01730, P01732, P10966
• Gene Alias: BB154331; Cd4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; CD4 molecule; CD4 receptor; CD4mut; CD8; CD8 antigen; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8A; CD8a molecule; CD8alpha; CD8b; CD8b molecule; CD8B1; CD8beta; L3T4; LEU2; Leu-2; Leu2 T-lymphocyte antigen; Ly-2; LY3; Ly-35; Ly-4; Ly-B; Lyt2; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); LYT3; MAL; OKT8 T-cell antigen; p32; P37; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T8 T-cell antigen; T-cell antigen Leu2; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-lymphocyte differentiation antigen T8/Leu-2
• Gene Symbols: CD4, CD8A, CD8B
• Host Species: Mouse
• Immunogen: Normal human blood lymphocytes, Human PHA blasts.
• Purification Method: Ion-exchange chromatography, Protein G
• Quantity: 50 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 920, 925, 926
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG1