CD4 Rat anti-Mouse, Brilliant Violet 510, Clone: GK1.5, BD Optibuild™

Catalog No.	Quantity
BDB743155	50 μg

Catalog No. BDB743155 Supplier BD Biosciences Supplier No. 743155

Description

Rat Monoclonal Antibody

The GK1.5 monoclonal antibody specifically binds to the mouse CD4 (L3T4) differentiation antigen. CD4 is expressed on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells. In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 has also been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. The GK1.5 antibody reportedly blocks binding of the RM4-5 and H129.19, but not RM4-4 mouse CD4-specific antibodies.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.

Specifications

• Antigen: CD4
• Classification: Monoclonal
• Clone: GK1.5
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 510
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Cd4, CD4 antigen, L3T4, Ly-4, T-cell surface antigen T4/Leu-3
• Host Species: Rat
• Immunogen: Mouse CTL clone V4
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2b κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov