CD4 Rat anti-Mouse, RB545, Clone: GK1.5, BD Horizon™

Rat Monoclonal Antibody

Supplier:  BD Biosciences 569281

Catalog No. BDB569281

Description

The GK1.5 monoclonal antibody specifically binds to the mouse CD4 (L3T4) differentiation antigen. CD4 is expressed on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells. In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 has also been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. The GK1.5 antibody reportedly blocks binding of the RM4-5 and H129.19, but not RM4-4 mouse CD4-specific antibodies.

CD4 Monoclonal antibody specifically detects CD4 in Mouse samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD4
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 12504
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
• Isotype: IgG2b κ

• Applications: Flow Cytometry
• Clone: GK1.5
• Conjugate: RB545
• Gene Alias: Cd4; CD4 antigen; L3T4; Ly-4; T-cell surface antigen T4/Leu-3
• Immunogen: Mouse CTL clone V4
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Form: Purified
• Excitation/Emission: Excitation Source: Blue 488 nm, Excitation Max: 496 nm, Emission Max: 545 nm

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov