Invitrogen™ CD40 Monoclonal Antibody (5C3), NovaFluor™ Yellow 610, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ H027T03Y03A

Catalog No. H027T03Y03A

Description

Description The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. 5C3 is reported to be used for activation of B cells in in vitro functional assays. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested The 5C3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.6 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Master mixes Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time. We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer. Whole Blood compatibility When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates. See the Bulk Lysis of Human Whole Blood protocol here. Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining. Viability dye compatib...

CD40 is a single-chain glycoprotein and a member of the tumor necrosis factor receptor (TNFR) family, exhibiting significant homology to the Hodgkin's disease-associated antigen, CD30. It is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells, as well as some epithelial cells, carcinomas, and lymphoid dendritic cells. Notably, CD40 is present on all B cells except plasma cells. CD40 plays a crucial role in regulating B cell development and maturation, inducing immunoglobulin isotype-switching, and protecting B cells from surface Ig-induced apoptosis when combined with other signals such as IL-4. It promotes proliferation and is essential for T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction between CD40 and its ligand CD154 (gp39) on T cells is vital for T-B cell crosstalk, costimulation, and immune regulation. Adaptor protein TNFR2 interacts with CD40, mediating signal transduction, while the AT-hook transcription factor AKNA is reported to regulate CD40 expression, which may be important for homotypic cell interactions. Additionally, the interaction between CD40 and its ligand is necessary for amyloid-beta-induced microglial activation, suggesting a role in the early pathogenesis of Alzheimer's disease. Two alternatively spliced transcript variants encoding distinct isoforms of CD40 have been identified. Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency, highlighting its importance in immune and inflammatory responses.

Specifications

• Antigen: CD40
• Classification: Monoclonal
• Concentration: 0.6 μg/Test
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P25942
• Gene Symbols: CD40
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 958
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: 5C3
• Conjugate: NovaFluor Yellow 610
• Gene: CD40
• Gene Alias: AI326936; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; Cd40; CD40 antigen; CD40 antigen (TNF receptor superfamily member 5); CD40 antigen, TNF receptor superfamily member 5; CD40 molecule; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; GP39; HIGM1; I79_006806; IGM; IMD3; Immunoglobulin M; ImmunoglobulinM; membrane protein CD40; MGC9013; p50; receptor for ligand CD154; sCD40; soluble CD40; T-BAM; T-cell differentiation antigen; Tnfrsf5; TRAP; tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Format: Liquid