CD40/TNFRSF5 Antibody (5D12), Janelia Fluor™ 549, Novus Biologicals™

Human Monoclonal Antibody

Supplier:  Novus Biologicals NBP281104JF549

Catalog No. NB016176

Description

CD40/TNFRSF5 Monoclonal specifically detects CD40/TNFRSF5 in Human, Cynomolgus Monkey samples. It is validated for Flow Cytometry, ELISA, Immunohistochemistry.

Specifications

• Antigen: CD40/TNFRSF5
• Classification: Monoclonal
• Conjugate: Janelia Fluor 549
• Formulation: 50mM Sodium Borate
• Gene Symbols: CD40
• Immunogen: Anti-CD40/TNFRSF5 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40/TNFRSF5 and selected for the ability to bind EBV-immortalized human B cells. The variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12.
• Quantity: 0.1 mL
• Research Discipline: Adaptive Immunity, Apoptosis, Asthma, B Cell Development and Differentiation Markers, Cancer, Hematopoietic Stem Cell Markers, Immunology, Signal Transduction, Stem Cell Markers
• Gene ID (Entrez): 958
• Target Species: Human, Cynomolgus Monkey
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry
• Clone: 5D12
• Dilution: Flow Cytometry, Immunohistochemistry
• Gene Alias: B cell surface antigen CD40, B-cell surface antigen CD40, Bp50B cell-associated molecule, CD40 antigen, CD40 molecule, TNF receptor superfamily member 5, CD40 type II isoform, CD40L receptor, CDw40, MGC9013, nerve growth factor receptor-related B-lymphocyte activation molecule, p50, TNFRSF5CD40 antigen (TNF receptor superfamily member 5), tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5
• Host Species: Human
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: ch5D12 binds specifically to recombinant and native human CD40/TNFRSF5 ectodomain and has been shown to also bind CD40/TNFRSF5 in cynomolgus and marmoset monkeys. The antibody requires residues within D1 and D1/B2 for binding (Hager et al, 2003) (D1 domain is crucial for CD40L binding). Binding characteristics are very similar between humanized ch5D12 and mu5D12. CD40/TNFRSF5 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40/TNFRSF5 binds to CD40L, and this interaction is involved in B-cell activation and proliferation, antigen-presenting cell (APC) activation, initiation of antigen-specific T-cell responses, immunoglobulin production, activation of effector macrophages isotype switching, homotypic adhesion and rescue from apoptosis.
• Content And Storage: Store at 4°C in the dark.
• Isotype: IgG4 κ