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CD42d Mouse Anti-Rat, Brilliant Violet 510, Clone: RPM.4, BD Optibuild™

Catalog No. BDB743618 Shop All BD Biosciences Products
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BDB743618 50 μg
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Catalog No. BDB743618 Supplier BD Biosciences Supplier No. 743618
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Description

Mouse Monoclonal Antibody

The RPM.4 monoclonal antibody specifically recognizes CD42d (Platelet glycoprotein V, GPV). CD42d is a 88-kDa type I transmembrane glycoprotein which noncovalently associates with GPIb and GPIX to form a receptor for von Willebrand factor on megakaryocytes and resting platelets. The binding sites for von Willebrand factor and thrombin have been localized to the GPIb chain of the GPI-b-V-IX complex. GPV is cleaved by thrombin to release a 70 kDa soluble fragment, which contains the epitope recognized by mAb RPM.4. Although the rat CD42d protein's structure is similar to that of human CD42d, the RPM.4 antibody does not cross-react with human platelets.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD42d
Classification Monoclonal
Clone RPM.4
Concentration 0.2mg/mL
Conjugate Brilliant Violet 510
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias PLGPV, Gp5, platelet glycoprotein V, GPV, Platelet glycoprotein 5
Host Species Mouse
Immunogen Wistar rat platelets
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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