CD42d Mouse Anti-Rat, BUV395, Clone: RPM.4, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 743623

Catalog No. BDB743623

Description

The RPM.4 monoclonal antibody specifically recognizes CD42d (Platelet glycoprotein V, GPV). CD42d is a 88-kDa type I transmembrane glycoprotein which noncovalently associates with GPIb and GPIX to form a receptor for von Willebrand factor on megakaryocytes and resting platelets. The binding sites for von Willebrand factor and thrombin have been localized to the GPIb chain of the GPI-b-V-IX complex. GPV is cleaved by thrombin to release a 70 kDa soluble fragment, which contains the epitope recognized by mAb RPM.4. Although the rat CD42d protein's structure is similar to that of human CD42d, the RPM.4 antibody does not cross-react with human platelets.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Specifications

• Antigen: CD42d
• Clone: RPM.4
• Conjugate: Brilliant Ultraviolet 395
• Gene Alias: PLGPV, Gp5, platelet glycoprotein V, GPV, Platelet glycoprotein 5
• Immunogen: Wistar rat platelets
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov