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Invitrogen™ CD43 Monoclonal Antibody (SP55)
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Catalog No. PIMA516339
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500 μL
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PIMA516339 500 μL
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Catalog No. PIMA516339 Supplier Invitrogen™ Supplier No. MA516339
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Description

Rabbit Monoclonal Antibody

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.

CD43, also known as leukosialin or sialophorin, is a transmembrane mucin-like protein characterized by a high negative charge. It is expressed on the surface of most hematopoietic cells, excluding resting B lymphocytes. CD43 serves both adhesive and anti-adhesive functions, contributing to a repulsive barrier that can interfere with cellular adhesion while also promoting leukocyte aggregation in certain contexts. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. Its expression decreases upon activation due to proteolytic processing. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. CD43 interacts with actin-binding proteins ezrin and moesin, playing a regulatory role in remodeling T-cell morphology and regulating cell-cell interactions during lymphocyte traffic. It enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. CD43 signaling induces the functionally active tumor suppressor p53 protein, but in cases of p53 and ARF deficiency, it can promote tumor proliferation and viability. CD43 is an important modulator of leukocyte functions, with dysfunctions associated with diseases such as Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD43
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
Classification Monoclonal
Clone SP55
Concentration Conc. Not Determined
Conjugate Unconjugated
Formulation PBS with 1% BSA and 0.1% sodium azide; pH 7.6
Gene SPN
Gene Accession No. P16150
Gene Alias 3E8 antigen; A630014B01Rik; B-cell differentiation antigen LP-3; CD43; CD43 cytoplasmic tail; CD43-ct; Galactoglycoprotein; GALGP; gpL115; Leukocyte sialoglycoprotein; leukosialin; leukosianin; LOC101120462; LOW QUALITY PROTEIN: leukosialin; Lsn; Lsn1; Ly48; Ly-48; Lymphocyte antigen 48; sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115; SPN; W3/13 antigen
Gene Symbols SPN
Host Species Rabbit
Immunogen Synthetic peptide derived from the human CD43.
Purification Method Protein A/G
Quantity 500 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 6693
Target Species Human
Content And Storage 4°C, do not freeze
Product Type Antibody
Form Liquid
Isotype IgG
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