CD43/Sialophorin Antibody (SPM503), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP232822F	Novus Biologicals NBP2328220.02MG	0.02 mg
NBP232822A	Novus Biologicals NBP2328220.1MG	0.1 mg
NBP232822B	Novus Biologicals NBP2328220.2MG	0.2 mg

Description

CD43/Sialophorin Monoclonal specifically detects CD43/Sialophorin in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: CD43/Sialophorin
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P16150
• Gene ID (Entrez): 6693
• Immunogen: Myeloblastic KG1 cells
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: SPM503
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Immunology
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: CD43 antigen, CD43), Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, sialophorin (gpL115, leukosialin, CD43)
• Gene Symbols: SPN
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.