Invitrogen™ CD45 Monoclonal Antibody (VPM18)

Catalog No.	Quantity
PIMA528385	100 μg

Catalog No. PIMA528385 Supplier Invitrogen™ Supplier No. MA528385

Description

Mouse Monoclonal Antibody

Mouse anti Sheep CD45, clone VPM18 recognizes the ovine homologue of human CD45 otherwise known as LCA (leucocyte common antigen), a type I transmembrane glycoprotein and member of the protein-tyrosine phosphatase (PTP) family, expressed at high levels on all nucleated cells of haematopoietic origin, and which exists in several isoforms generated by the alternative splicing.

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Specifications

• Antigen: CD45
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
• Classification: Monoclonal
• Clone: VPM18
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide
• Gene: PTPRC
• Gene Accession No.: 0
• Gene Alias: B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220
• Gene Symbols: PTPRC
• Host Species: Mouse
• Immunogen: Sheep T lymphocytes.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 101102917
• Target Species: Sheep
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1