CD45 Mouse, Clone: 2B11 + PD7/26, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 2 publications

Catalog Number	Mfr. No.	Quantity
NBP234287F	Novus Biologicals NBP2342870.02MG	0.02 mg
NBP234287A	Novus Biologicals NBP2342870.1MG	0.1 mg
NBP234287B	Novus Biologicals NBP2342870.2MG	0.2 mg

Description

CD45 Monoclonal specifically detects CD45 in Human, Canine samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: CD45
• Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Canine
• Gene Accession No.: P08575
• Gene ID (Entrez): 5788
• Immunogen: Isolated neoplastic cells from T cell lymphoma (2B11); and human peripheral blood lymphocytes maintained in T cell growth factor (PD7/26)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: 2B11 + PD7/26
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, MAP Kinase Signaling, Mast Cell Markers, Mesenchymal Stem Cell Markers, Microglia Markers, Modulation of DNA Pools, Myeloid Cell Markers, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
• Gene Symbols: PTPRC
• Isotype: IgG
• Purification Method: Protein A purified
• Test Specificity: Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.