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CD45 Rat anti-Mouse, RB545, Clone: 30-F11, BD Horizon™

Catalog No. BDB569257 Shop All BD Biosciences Products
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BDB569257 0.1 mg
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Catalog No. BDB569257 Supplier BD Biosciences Supplier No. 569257
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Description

Rat Monoclonal Antibody

The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family, where the intracellular carboxy-terminal region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated as A, B, and C, respectively). CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.

CD45 Monoclonal antibody specifically detects CD45 in Mouse samples. It is validated for Flow Cytometry
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD45
Applications Flow Cytometry
Classification Monoclonal
Clone 30-F11
Concentration 0.2 mg/mL
Conjugate RB545
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias Ptprc, LCA, Leukocyte common antigen, T200, Ly-5, Lyt-4
Host Species Rat
Immunogen Mouse Thymus / Spleen
Purification Method Affinity Purified
Quantity 0.1 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 19264
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
Form Purified
Isotype IgG2b κ
Excitation/Emission Excitation Source: Blue 488 nm, Excitation Max: 496 nm, Emission Max: 545 nm
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