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CD45R (B220) Monoclonal Antibody (HIS24), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

$172.00

Specifications

Antigen CD45R (B220)
Clone HIS24
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
46-046-082
Encompass_Preferred
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LIFE TECHNOLOGIES
46046082
100 μg
Each of 1 for $172.00
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Description

Description

Description: The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. CD45, also known as Leukocyte Common Antigen (LCA), has tyrosine phosphatase enzymatic activity in its cytoplasmic portion and plays an important role in activation of lymphocytes. Applications Reported: This HIS24 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIS24 antibody has been tested by flow cytometric analysis of rat splenocytes. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation.

Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser CD45R is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.
Specifications

Specifications

CD45R (B220)
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
24699
IgG2b κ
Affinity Chromatography
Antibody
HIS24
Flow Cytometry
PerCP-eFluor 710
Mouse
Rat
P04157
Ptprc
Primary
4° C, store in dark, DO NOT FREEZE!
Ptprc
SDS
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Documents

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