CD45RB Mouse anti-Human, Clone: PTPRC/1147, Abnova™

Catalog No.	Quantity
89-933-834	100 μg

Catalog No. 89-933-834 Supplier Abnova Corporation Supplier No. MAB13025

Description

Mouse monoclonal antibody raised against full length recombinant human CD45RB.

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq]

Specifications

• Antigen: CD45RB
• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (PFA fixed), Western Blot
• Classification: Monoclonal
• Clone: PTPRC/1147
• Conjugate: Unconjugated
• Description: Mouse monoclonal antibody raised against full length recombinant human CD45RB.
• Dilution: Flow Cytometry (0.5-1 ug/million cells in 0.1 mL) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined
• Formulation: In 10mM PBS (0.05% BSA, 0.05% sodium azide).
• Gene Alias: B220/CD45/CD45R/GP180/LCA/LY5/T200
• Gene Symbols: PTPRC
• Host Species: Mouse
• Immunogen: Recombinant protein corresponding to full length of human CD45RB.
• Purification Method: Protein A/G purification
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 5788
• Test Specificity: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
• Target Species: Human
• Content And Storage: Store at 4°C.
• Form: Liquid
• Isotype: IgG1 κ