CD45RC Mouse Anti-Rat, BUV395, Clone: OX-22, BD Optibuild
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 742492
The OX-22 antibody reacts with CD45RC on pre-B lymphocytes, B cells, CD8+ T suppressor/cytotoxic cells, and a subset of CD4+ T helper (Th) lymphocytes. It weakly reacts with thymocytes. CD45RC is a high-molecular-weight isoform of CD45 (Leukocyte Common Antigen); its level of expression distinguishes subpopulations of CD4+ T cells with Th1-like and Th2-like effector functions. Levels of expression of CD45RC have also been reported to distinguish resting from activated T cells at various stages of maturation. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|PHA-activated rat lymph node cells|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.|
|Brilliant Ultraviolet 395|
|Mouse BALB/c IgG1, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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