Invitrogen™ CD46 Monoclonal Antibody (8E2), PerCP-eFluor™ 710, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-113-0754	Invitrogen™ 46046942	100 Tests

Description

Description: The 8E2 monoclonal antibody reacts with human CD46 (membrane cofactor protein). CD46 is a transmembrane glycoprotein consisting of four regularly expressed isoforms, giving an electrophoretic profile of two heterogeneous protein species of 51-58 kDa (lower band) and 59-68 kDa (upper band). CD46 is expressed on the majority of nucleated cells, and functions as a regulator of complement function by binding complement components C3b and C4b, which serves to protect the host from autologous complement attack. CD46 has been called a pathogen magnet as it is known to play a role in the entry of several pathogens including strains of measles virus, human herpes virus type 6, group A streptococci and Neisseria. Cross-linking TCR on naive human peripheral blood CD4+ T cells together with CD46, rather than CD28, induces more robust proliferation than achieved with stimulation by the costimulatory molecule CD28, as well as other qualitative differences. There is evidence to suggest that stimulation of TCR and CD46 activates natural CD4+CD25+ T regs, which are non-proliferating, but produce high amounts of granzyme A, and kill activated CD4+, CD8+, CD14+ monocytes, immature dendritic cells and mature dendritic cells. Applications Reported: This 8E2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 8E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human perip...

CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.

Specifications

• Antigen: CD46
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: PerCP-eFluor 710
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P15529
• Gene ID (Entrez): 4179
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: 8E2
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human
• Gene: CD46
• Gene Alias: AHUS2; antigen identified by monoclonal antibody TRA-2-10; brain microvascular membrane cofactor; brain microvascular membrane cofactor protein CD46; Cd46; CD46 antigen, complement regulatory protein; CD46 complement regulatory protein; CD46 molecule; CD46 molecule, complement regulatory protein; CD46 splice variant; complement membrane cofactor protein; complement regulatory protein; Mcp; MCP; complement activation regulator; measles virus receptor; membrane cofactor protein; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); membrane cofactor protein variant 10; membrane cofactor protein variant 7; membrane cofactor protein variant 8; membrane cofactor protein variant 9; MGC26544; MIC10; soluble membrane cofactor protein variant 11; soluble membrane cofactor protein variant 12; soluble membrane cofactor protein variant 13; TLX; TRA2.10; trophoblast leucocyte common antigen; trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen
• Gene Symbols: CD46
• Product Type: Antibody