CD47 Antibody (IAP/964 + B6H12.2), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24440900	Novus Biologicals NBP2444090.02MG	0.02 mg
NBP24440901	Novus Biologicals NBP2444090.1MG	0.1 mg
NBP24440902	Novus Biologicals NBP2444090.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

CD47 Monoclonal specifically detects CD47 in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen, Functional, Flow (Cell Surface), Immunohistochemistry-Paraffin (Negative).

Specifications

• Antigen: CD47
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Immunology, Signal Transduction
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: antigen identified by monoclonal 1D8, Antigenic surface determinant protein OA3, CD47 antigen, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, CD47 molecule, IAPintegrin associated protein, Integrin-associated protein, leukocyte surface antigen CD47, MER6integrin-associated signal transducer, OA3, Protein MER6, Rh-related antigen
• Gene Symbols: CD47
• Isotype: IgG
• Purification Method: Protein A or G purified
• Test Specificity: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

• Clone: IAP/964 + B6H12.2
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Flow (Cell Surface) 1:10-1:1000, Immunofluorescence 1 - 2 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Gene Accession No.: Q08722
• Gene ID (Entrez): 961
• Immunogen: Recombinant human IAP protein (IAP/964); Intact CD47 purified from placenta (B6H12.2)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

For Research Use Only