CD47 Mouse anti-Human, Brilliant Violet 421, Clone: B6H12, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 566255
The B6H12 monoclonal antibody specifically binds to CD47, a 42-52 kDa N-linked glycan protein. CD47 antigen, also known as integrin-associated protein (IAP), is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. CD47 may play a role as a signal transducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells. The B6H12 antibody is capable of blocking phagocytosis of microparticles by peripheral blood granulocytes. It has also been reported to induce proliferation of CD3-activated T cellsThe antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
|Brilliant Violet 421|
|Mouse BALB/c IgG1, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|IAP, CDw149, gp42, MER6, Neurophilin, OA3, Rh-related antigen|
|Purified human placental RGD-binding proteins|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV421 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV421 were|
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