Invitrogen™ CD47 Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA547215

Catalog No. PIPA547215

Description

In direct ELISAs, less than 15% cross-reactivity with recombinant human CD47 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

CD47, known as integrin-associated protein (IAP), is a glycosylated transmembrane glycoprotein with five domains, expressed widely across hematopoietic cells like T and B cells, monocytes, platelets, and erythrocytes, as well as non-hematopoietic cells. It interacts with integrins such as CD51/CD61 and CD41/CD61, and serves as a receptor for thrombospondin, mediating bi-directional signaling that affects neural synaptic activity and macrophage phagocytosis. CD47 acts as a ligand for CD172a (SIRP alpha), an inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. This interaction influences cell migration, B cell adhesion, T cell activation, and neuronal development, particularly in synapse-rich brain and retina regions. It also modulates chondrocyte responses to mechanical signals. T cell expression of CD47 can lead to activation or apoptosis in the presence of thrombospondin. Monoclonal antibody stimulation of CD47 has been shown to induce CD4+CD25- suppressive activity and increase Foxp3 expression. CD47's role in membrane transport, signal transduction, and its broad tissue distribution highlight its significance in various physiological processes.

Specifications

• Antigen: CD47
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CD47
• Gene Alias: 9130415E20Rik; AA407862; AI848868; antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; AW108519; B430305P08Rik; Cd47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen/integrin-associated protein; CD47 glycoprotein; CD47 molecule; CD47/IAP; IAP; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; Itgp; Leukocyte surface antigen CD47; MER6; OA3; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16423, 29364
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: P97829, Q61735
• Gene Symbols: CD47
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD47 N-terminal IgV-like Gln19-Pro158.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG