Invitrogen™ CD47 Recombinant Rabbit Monoclonal Antibody (BLR131H)

Rabbit Recombinant Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA544451	Invitrogen™ MA544451	100 μL
PIMA544452	Invitrogen™ MA544452	10 μL

Description

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

Specifications

• Antigen: CD47
• Concentration: 50 μg/mL
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: BBS with 0.1% BSA and 0.09% sodium azide; pH 8.2
• Gene Accession No.: Q08722
• Gene ID (Entrez): 961
• Immunogen: Recombinant protein representing the extracellular domain of Human leukocyte surface antigen CD47 (residues 19-142).
• Product Type: Antibody

• Clone: BLR131H
• Content And Storage: 4°C
• Classification: Recombinant Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human
• Gene: CD47
• Gene Alias: 9130415E20Rik; AA407862; AI848868; antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; AW108519; B430305P08Rik; Cd47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen/integrin-associated protein; CD47 glycoprotein; CD47 molecule; CD47/IAP; IAP; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; Itgp; Leukocyte surface antigen CD47; MER6; OA3; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47
• Gene Symbols: CD47
• Primary or Secondary: Primary
• Purification Method: purified