CD49a Mouse anti-Human, Brilliant Violet 650, Clone: SR84, BD Optibuild™
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 742360
The SR84 monoclonal antibody specifically recognizes CD49a (CD49 antigen-like family member A). CD49a is a ñ200 kDa type I transmembrane molecule that is encoded by ITGA1 ( /Integrin subunit alpha 1). CD49a is also known as very late antigen 1αsubunit (VLA-1) of the integrin family of cell adhesion molecules. CD49a associates with the integrinβ1 subunit (CD29) to form theα1/β1 heterodimer (CD49a/CD29), and serves as receptor for collagen and laminin-1. It is expressed on activated T cells, monocytes, neuronal cells and smooth muscle cells. CD49a has been reported to play a role in cell attachment during the development of both the central and peripheral nervous systems.The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Integrin α1, Integrin alpha-1, ITGA1, ITA1, VLA-1, VLA1|
|P-Selectin IgG1 Fusion|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV650 under optimal conditions that minimize unconjugated dye and antibody.|
|Brilliant Violet 650|
|Mouse IgG1, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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