CD49b Mouse anti-Human, Brilliant Violet 605, Clone: 12F1, BD Optibuild™

Catalog No.	Quantity
BDB743203	50 μg

Catalog No. BDB743203 Supplier BD Biosciences Supplier No. 743203

Description

Mouse Monoclonal Antibody

The 12F1 monoclonal antibody specifically binds to CD49b. CD49b is also known as the integrinα2 chain (VLA-α2), a member of the integrin family of extracellular matrix and cell-cell adhesion receptors. VLA-α2 is non-covalently associated with VLA-β1 chain (CD29) in the VLA-2 complex.α2 is expressed on numerous cell types including activated T cells, platelets, and long-term cultivated T cells.α2β1 is a well-established receptor for collagen. This antibody is useful for studies of integrin expression and function.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.

Specifications

• Antigen: CD49b
• Classification: Monoclonal
• Clone: 12F1
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 605
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Integrin α2 chain, ITGA2, VLA-2 alpha, Platelet GPIa, Collagen receptor, BR
• Host Species: Mouse
• Immunogen: Human PBMC and Platelets
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ