CD49d Mouse anti-Human, Brilliant Violet 786, Clone: 9F10, BD Optibuild™

Catalog No.	Quantity
BDB744588	50 μg

Catalog No. BDB744588 Supplier BD Biosciences Supplier No. 744588

Description

Mouse Monoclonal Antibody

The 9F10 monoclonal antibody specifically reacts with the integrinα4 chain, that is expressed as a heterodimer with either of twoβintegrin subunits,β1 (CD29) orβ7. Theα4β1 integrin (VLA-4) is expressed on lymphocytes, monocytes, thymocytes, NK cells, and several B- and T-cell lines, and mediates binding to VCAM-1 (CD106) and the CS-1 region of fibronectin. Theα4β7 integrin has a similar tissue distribution, except it is found on only a small subpopulation of thymocytes. Integrinα4β7 also binds fibronectin and VCAM-1, and has been shown in the mouse to preferentially bind the mucosal vascular addressin molecule, MAdCAM-1. This antibody is useful for studies of the expression by and function of cells that expressα4 chain-containing integrins. This clone cross-reacts with a subset of peripheral blood lymphocytes, monocytes, and some granulocytes of baboon and both rhesus and cynomolgus macaque monkeys. The distribution on leukocytes is similar to that observed with human peripheral blood leukocytes.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.

Specifications

• Antigen: CD49d
• Classification: Monoclonal
• Clone: 9F10
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 786
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Integrin α4 chain, Integrin alpha 4, ITGA4, IA4, alpha 4 subunit of VLA-4
• Host Species: Mouse
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ