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CD5 Mouse anti-Human, Brilliant Violet 650, Clone: L17F12, BD Optibuild™

Catalog No. BDB742551 Shop All BD Biosciences Products
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BDB742551 50 μg
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Catalog No. BDB742551 Supplier BD Biosciences Supplier No. 742551
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Description

Mouse Monoclonal Antibody

The L17F12 monoclonal antibody specifically binds to CD5 which is also known as Lymphocyte antigen T1 (T1) or LEU1/Leu-1. CD5 is an ñ67 kDa single-chain, type 1 transmembrane glycoprotein expressed on most thymocytes, the majority of peripheral T lymphocytes and a subpopulation of B cells. CD5+ B cells produce polyreactive antibodies (mostly IgM). This CD5-specific antibody also reacts with many T-cell leukemias and lymphomas and most Ig+ B-cell chronic lymphoblastic leukemia (B-CLL) cells. CD72 has been shown to be the natural ligand for CD5.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD5
Classification Monoclonal
Clone L17F12
Concentration 0.2mg/mL
Conjugate Brilliant Violet 650
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias LEU1, Leu-1, Lymphocyte antigen T1, T1, LY1, Tp67
Host Species Mouse
Immunogen Human T-cell acute lymphoblastic leukemia (T-ALL) cells
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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