Invitrogen™ CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), Biotin, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-115-89	Invitrogen™ 13051982	100 μg

Description

Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin. Applications Reported: This 23C6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 23C6 antibody has been tested by flow cytometric analysis of peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.

Specifications

• Antigen: CD51/CD61 (Integrin alpha v beta 3)
• Concentration: 0.5 mg/mL
• Applications: Flow Cytometry
• Conjugate: Biotin
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05106, P06756
• Gene ID (Entrez): 3685, 3690
• Primary or Secondary: Primary
• Purification Method: Affinity Chromatography

• Clone: 23C6
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human
• Gene: ITGAV
• Gene Alias: 1110004F14Rik; 2610028E01Rik; alpha v integrin; antigen CD61; antigen identified by monoclonal antibody L230; BDPLT16; BDPLT2; Cd51; CD61; D430040G12Rik; Dnmt3l-ps1 pseudogene; GP3A; GPIIIa; GT; INGRB3; integrin; integrin alpha V; integrin alpha-5 beta-3; integrin alpha-V; Integrin alpha-V heavy chain; Integrin alpha-V light chain; integrin alphaVbeta3; Integrin beta; integrin beta 3; integrin beta 3 (Cd61); Integrin beta3; integrin beta-3; integrin subunit alpha V; integrin subunit beta 3; integrin, alpha V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin, beta 3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITGAV; Itgb3; MSK8; platelet gpIIIa; platelet membrane glycoprotein IIIa; Vitronectin receptor; vitronectin receptor alpha polypeptide (VNRA); vitronectin receptor alpha subunit; vitronectin receptor subunit alpha; VNRA; VTNR
• Gene Symbols: ITGAV, ITGB3
• Product Type: Antibody