CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 16051981

Catalog No. 50-143-44

Description

Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. CD51, an ∽120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin. Applications Reported: This 23C6 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. 23C6 has also been reported in blocking of some adhesive processes. Applications Tested: This 23C6 antibody has been tested by flow cytometric analysis of human melanoma cell line and peripheral blood. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.

CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.

Specifications

• Antigen: CD51/CD61 (Integrin alpha v beta 3)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P05106, P06756
• Gene Symbols: ITGAV, ITGB3
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3685, 3690
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Neutralization
• Clone: 23C6
• Conjugate: Functional Grade
• Gene: ITGB3
• Gene Alias: 1110004F14Rik; 2610028E01Rik; alpha v integrin; antigen CD61; antigen identified by monoclonal antibody L230; BDPLT16; BDPLT2; Cd51; CD61; D430040G12Rik; Dnmt3l-ps1 pseudogene; GP3A; GPIIIa; GT; INGRB3; integrin; integrin alpha V; integrin alpha-5 beta-3; integrin alpha-V; Integrin alpha-V heavy chain; Integrin alpha-V light chain; integrin alphaVbeta3; Integrin beta; integrin beta 3; integrin beta 3 (Cd61); Integrin beta3; integrin beta-3; integrin subunit alpha V; integrin subunit beta 3; integrin, alpha V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin, beta 3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITGAV; Itgb3; MSK8; platelet gpIIIa; platelet membrane glycoprotein IIIa; Vitronectin receptor; vitronectin receptor alpha polypeptide (VNRA); vitronectin receptor alpha subunit; vitronectin receptor subunit alpha; VNRA; VTNR
• Host Species: Mouse
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.