Invitrogen™ CD52 Monoclonal Antibody (CF1D12), Super Bright™ 702, eBioscience™

Catalog No.	Quantity
67-052-942	100 Tests

Catalog No. 67-052-942 Supplier Invitrogen™ Supplier No. 67052942

Description

Mouse Monoclonal Antibody

This CF1D12 monoclonal antibody reacts with human CD52, a cell surface glycoprotein expressed in the immune and reproductive systems. CF1D12 is thought to recognize the carbohydrate region of CD52, not the protein core of the molecule. Tissue-specific modifications of the glycan structure may alter epitope recognition in certain applications. For example, this CF1D12 clone has been reported to recognize human sperm glycoforms of CD52 by western blot, but not by flow cytometry. The immunogen used to generate this antibody was human CD52. Applications Reported: This CF1D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This CF1D12 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interac...

CD52, also known as CAMPATH-1 antigen, is a glycoprotein that is expressed on the surface of various immune cells, including T and B lymphocytes, natural killer cells, and monocytes. It is involved in the regulation of immune responses and is being studied as a potential therapeutic target in various diseases, including multiple sclerosis, chronic lymphocytic leukemia, and autoimmune disorders. CD52 is the target of alemtuzumab, a monoclonal antibody that is used to treat these conditions by depleting immune cells that express CD52.

Specifications

• Antigen: CD52
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: CF1D12
• Concentration: 5 μL/Test
• Conjugate: Super Bright 702
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: CD52
• Gene Accession No.: P31358
• Gene Alias: AI463198; B7; B7-Ag; cambridge pathology 1 antigen; CAMPATH-1; CAMPATH-1 antigen; Cd52; CD52 antigen; CD52 antigen (CAMPATH-1 antigen); CD52 molecule; CDW52; CDW52 antigen (CAMPATH-1 antigen); CLS1; Epididymal secretory protein E5; epididymis secretory sperm binding protein Li 171mP; he5; HEL-S-171mP; human epididymis-specific protein 5; Lymphocyte differentiation antigen B7; Mb7
• Gene Symbols: CD52
• Host Species: Mouse
• Immunogen: Human CD52.
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1043
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG3 κ

Safety and Handling

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