CD53 Antibody (63-5A3), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24460800	Novus Biologicals NBP2446080.02MG	0.02 mg
NBP24460801	Novus Biologicals NBP2446080.1MG	0.1 mg
NBP24460802	Novus Biologicals NBP2446080.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

CD53 Monoclonal specifically detects CD53 in Human samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Functional, Immunofluorescence.

Specifications

• Antigen: CD53
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, SDS-Page, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Immunology
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 963
• Immunogen: Human Sezary cells
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: 63-5A3
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: antigen MOX44 identified by monoclonal MRC-OX44, CD53 antigentetraspanin-25, CD53 glycoprotein, CD53 molecule, CD53 tetraspan antigen, cell surface antigen, Cell surface glycoprotein CD53, MOX44transmembrane glycoprotein, Tetraspanin-25, tspan-25, TSPAN25leukocyte surface antigen CD53
• Gene Symbols: CD53
• Isotype: IgG2b κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.

For Research Use Only