Invitrogen™ CD54 (ICAM-1) Monoclonal Antibody (HA58), NovaFluor™ Blue 610-70S, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ H035T02B06A

Catalog No. H035T02B06A

Description

Description ;The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response. ; ;This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. ; ;Applications Tested ;The HA58 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 5 μL (0.6 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. ; ;Master mixes ; Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time. ; We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer. ; ;Whole Blood compatibility ; When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates.<br...

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

Specifications

• Antigen: CD54 (ICAM-1)
• Classification: Monoclonal
• Concentration: 0.6 μg/Test
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05362
• Gene Symbols: ICAM1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3383
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: HA58
• Conjugate: NovaFluor Blue 610-70S
• Gene: ICAM1
• Gene Alias: BB2; CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM; Icam1; ICAM-1; ICAM-1; CD54 homolog; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; intercellular adhesion molecule-1 precursor; Ly 47; Ly-47; major group rhinovirus receptor; MALA2; MALA-2; MyD10; P3.58; sCD54; soluble CD54; Surface antigen of activated B cells
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Format: Liquid