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CD54 (ICAM-1) Rat anti-Mouse, Clone: YN1/1.7.4, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$52.75 - $194.00

Specifications

Antigen CD54 (ICAM-1)
Clone YN1/1.7.4
Target Species Mouse
Conjugate Unconjugated
Isotype IgG2b, kappa
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Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-126-47 Non-distribution product as customer accommodation.
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Invitrogen
14054181
50 μg
Each for $52.75
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50-126-48 Non-distribution product as customer accommodation.
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Invitrogen
14054182
100 μg
Each for $107.00
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50-126-49 Non-distribution product as customer accommodation.
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Invitrogen
14054185
500 μg
Each for $194.00
Add to cart
 
Description

Description

Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody. Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. YN1/1.7.4 has also been reported in blocking of CD54 in functional studies. (Please use Functional Grade purified YN1/1.7.4, cat. 16-0541, in functional assays.). Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
Specifications

Specifications

CD54 (ICAM-1)
Mouse
IgG2b, kappa
Monoclonal
Liquid
Antibody
4° C
YN1/1.7.4
Unconjugated
Flow Cytometry, Functional Assay, Immunohistochemistry, Neutralization
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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