Invitrogen™ CD55 Monoclonal Antibody (583905)

Catalog No.	Quantity
PIMA524264	100 μg

Catalog No. PIMA524264 Supplier Invitrogen™ Supplier No. MA524264

Description

Rat Monoclonal Antibody

In direct ELISAs, no cross-reactivity with recombinant human CD55 or recombinant mouse CD97 is observed. Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can regulate complement when bound to CD97. In tumors, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis. CD55 is a 70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells, the apparent molecular weight is somewhat larger for CD55 and has a substantial content of O-glycans. CD55 binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. CD55 carries the Cromer related blood group antigens. Two alternatively spliced transcripts encoding CD55 have been identified, and the predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. CD55 has been studied to have a role in prostate cancer growth and survival.

Specifications

• Antigen: CD55
• Applications: Flow Cytometry, Western Blot
• Classification: Monoclonal
• Clone: 583905
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5% trehalose and No Preservative
• Gene: CD55
• Gene Accession No.: Q61475
• Gene Alias: CD55; CD55 antigen; CD55 molecule (Cromer blood group); CD55 molecule, decay accelerating factor for complement; CD55 molecule, decay accelerating factor for complement (Cromer blood group); Cd55a; Complement decay-accelerating factor; complement decay-accelerating factor, GPI-anchored; complement-glycosylphosphatidylinositol; CR; CROM; Cromer blood group; Daf; Daf1; DAF-GPI; decay accelarating factor 1; decay accelerating factor 1; decay accelerating factor GPI-form; decay-accelarating factor; glycosylphophatidylinositol-anchored form; GPI anchor addition signal; GPI-DAF; RP11-357P18.1; TC
• Gene Symbols: CD55
• Host Species: Rat
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD55/DAF Met1-Pro359.
• Purification Method: Protein A/G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 13136
• Target Species: Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2a

Frequently Asked Questions (FAQs)

Have you tested the CD55 Monoclonal Antibody (583905) (Cat. No. MA5-24264) in ICC/IF application?

Unfortunately, this antibody has not been internally verified for ICC/IF, and therefore we cannot guarantee that it will work for this application.

Alternatively, we offer CD55 Recombinant Rabbit Monoclonal Antibody (76) (Cat. No. MA5-29678) and CD55 Polyclonal Antibody (Cat. No. PA5-88462) that target mouse and have been verified for ICC/IF.