Invitrogen™ CD55 Polyclonal Antibody

Catalog No.	Quantity
PIPA588462	100 μL

Catalog No. PIPA588462 Supplier Invitrogen™ Supplier No. PA588462

Description

Rabbit Polyclonal Antibody

Immunogen sequence: PDVPNAQPAL EGRTSFPEDT VITYKCEESF VKIPGEKDSV ICLKGSQWSD IEEFCNRSCE VPTRLNSASL KQPYITQNYF PVGTVVEYEC RPGYRREPSL SPKLTCLQNL KWSTAVEFCK KKSCPNPGEI RNGQIDVPGG ILFGATISFS CNTGYKLFGS TSSFCLISGS SVQWSDPLPE CREIYCPAPP QIDNGIIQGE RDHYGYRQSV TYACNKGFTM IGEHSIYCTV NNDEGEWSGP PPECRGKSLT SKVPPTVQKP T; Positive Samples: HeLa, A-549, DU145, MCF7, 293T; Cellular Location: Cell membrane, GPI-anchor, Lipid-anchor, Single-pass type I membrane protein.

CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can regulate complement when bound to CD97. In tumors, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis. CD55 is a 70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells, the apparent molecular weight is somewhat larger for CD55 and has a substantial content of O-glycans. CD55 binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. CD55 carries the Cromer related blood group antigens. Two alternatively spliced transcripts encoding CD55 have been identified, and the predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. CD55 has been studied to have a role in prostate cancer growth and survival.

Specifications

• Antigen: CD55
• Applications: ELISA, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1.36 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene: CD55
• Gene Accession No.: P08174, Q61475
• Gene Alias: CD55; CD55 antigen; CD55 molecule (Cromer blood group); CD55 molecule, decay accelerating factor for complement; CD55 molecule, decay accelerating factor for complement (Cromer blood group); Cd55a; Complement decay-accelerating factor; complement decay-accelerating factor, GPI-anchored; complement-glycosylphosphatidylinositol; CR; CROM; Cromer blood group; Daf; Daf1; DAF-GPI; decay accelarating factor 1; decay accelerating factor 1; decay accelerating factor GPI-form; decay-accelarating factor; glycosylphophatidylinositol-anchored form; GPI anchor addition signal; GPI-DAF; RP11-357P18.1; TC
• Gene Symbols: CD55
• Host Species: Rabbit
• Immunogen: Recombinant protein (or fragment).
• Purification Method: Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 13136, 1604, 64036
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Frequently Asked Questions (FAQs)

Have you tested the CD55 Monoclonal Antibody (583905) (Cat. No. MA5-24264) in ICC/IF application?

Unfortunately, this antibody has not been internally verified for ICC/IF, and therefore we cannot guarantee that it will work for this application.

Alternatively, we offer CD55 Recombinant Rabbit Monoclonal Antibody (76) (Cat. No. MA5-29678) and CD55 Polyclonal Antibody (Cat. No. PA5-88462) that target mouse and have been verified for ICC/IF.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov