CD59 Antibody (SPM616), Alexa Fluor™ 750, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP247821AF750

Catalog No. NP247821A70

Description

CD59 Monoclonal specifically detects CD59 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: CD59
• Classification: Monoclonal
• Conjugate: Alexa Fluor 750
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: CD59
• Immunogen: Recombinant full-length human CD59 protein (Uniprot: P13987)
• Quantity: 0.1 mL
• Gene ID (Entrez): 966
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: SPM616
• Dilution: Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence
• Gene Alias: 16.3A5, 1F5, 1F5 antigen, 20 kDa homologous restriction factor, CD59 antigen, CD59 antigen, complement regulatory protein, CD59 glycoprotein, CD59 molecule, complement regulatory protein, EJ16, EJ30, EJ30, EL32 and G344), EL32, FLJ38134, FLJ92039, G344, HRF20, HRF-20, human leukocyte antigen MIC11, Ly-6-like protein, lymphocytic antigen CD59/MEM43, MACIF, MAC-inhibitory protein, MAC-IP, MEM43, MEM43 antigen, membrane attack complex (MAC) inhibition factor, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MGC2354, MIC11MSK21, MIN1, MIN2, MIN3, MIRL, p18-20, protectin, surface anitgen recognized by monoclonal 16.3A5, T cell-activating protein
• Host Species: Mouse
• Purification Method: Protein G purified
• Primary or Secondary: Primary
• Test Specificity: Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This monoclonal antibody is useful for study on GPI-anchored proteins, PNH and CD59 functions. CD59 is widely distributed on cells in all tissues. The expression of CD59 on erythrocytes is important for their survival.
• Content And Storage: Store at 4°C in the dark.