Invitrogen™ CD61 Monoclonal Antibody (F11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA180862

Catalog No. PIMA180862

Description

For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti Rat CD61 antibody, clone F11 recognizes the rat CD61 cell surface antigen, also known as the integrin b3 chain.

CD61, also known as GPIIIa or ITGB3, is a 105 kDa glycoprotein expressed on activated T cells, granulocytes, megakaryocytes, platelets, and their precursors. It plays a crucial role in platelet aggregation and functions as a receptor for fibrinogen, fibronectin, von Willebrand factor, vitronectin, and thrombospondin. CD61 forms heterodimeric complexes by associating non-covalently with integrin alpha subunits: alphaV (CD51) to create the Vitronectin Receptor and alphaIIb (CD41) to form gpIIb/IIIa. These complexes are responsible for adhesion to extracellular matrix components, facilitating cell adhesion and cell-surface mediated signaling. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, and osteoclasts in association with CD51. The protein product of CD61 is composed of an alpha chain and a beta chain, which can combine with multiple partners to form different integrins. Its involvement in cell adhesion and signaling underscores its importance in normal physiological processes. Dysfunction of CD61 is associated with diseases such as Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder, highlighting its critical role in hemostasis and platelet function.

Specifications

• Antigen: CD61
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: P05106
• Gene Symbols: ITGB3
• Immunogen: Bone cell suspension from SD rats.
• Quantity: 250 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
• Clone: F11
• Conjugate: Unconjugated
• Gene: ITGB3
• Gene Alias: antigen CD61; BDPLT16; BDPLT2; beta3 integrin; CD61; EGK_08849; glycoprotein GPIIIa; glycoprotein IIIa; GP3A; GPIIIa; GT; INGRB3; integrin beta 3; integrin beta 3 (Cd61); integrin beta-3; integrin beta-3 subunit; integrin subunit beta 3; integrin, beta 3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITGB3; platelet glycoprotein IIIa; platelet glycoprotein IIIa integrin subunit beta 3; platelet gpIIIa; platelet membrane glycoprotein IIIa
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 29302, 3690
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid