Invitrogen™ CD62L (L-Selectin) Monoclonal Antibody (DREG.55), FITC, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ BMS121FI

Catalog No. 50-177-67

Description

Description: The antibody can be used to stain acetone-fixed cryostat sections or cell smears. BMS121 is not suitable for paraffin sections but is suitable as primary antibody in staining for FACS analysis. BMS121FI can be used for direct staining of cells, BMS121BT can be used in a 2-step procedure using the biotin-(strept)avidin system. BMS121 has not been tested in functional assays but is suitable for ELISA application. Leukocyte-Endothelial Cell Adhesion Molecule-1 belongs to the selectin family of adhesion molecule. Together with E-selectin, P-selectin and L-selectin mediates the initial interactions of leukocytes with endothelial cells. Selectins guide non-activated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer. L-selectin in this aspect mediates rolling of PMN's on endothelial cells. The potential binding partners of L-selectin carry a negative charge, probably a sialic acid and/or sulphate, and may contain mannose and fucose. In addition, L-selectin may also interact with E-selectin which is expressed on cytokine-activated endothelial cells. L-selectin is constitutively expressed on most leukocytes in a seemingly functional form. It is required for the binding of lymphocytes to the high endothelial venules of peripheral lymph nodes and for the invasion of neutrophils into sites of inflammation. When neutrophils are activated, L-selectin is shed by proteolytic cleveage near the transmembrane span. ...

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

Specifications

• Antigen: CD62L (L-Selectin)
• Classification: Monoclonal
• Concentration: 10 μL/Test
• Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.2-7.4
• Gene Accession No.: P14151
• Gene Symbols: SELL
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6402
• Content And Storage: 4°C, store in dark
• Form: Liquid

• Applications: Flow Cytometry
• Clone: DREG.55
• Conjugate: FITC
• Gene: SELL
• Gene Alias: A.11; AI528707; CD62 antigen-like family member L; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LECAM1; LECAM-1; LEU8; Leu-8; Leukocyte adhesion molecule 1; leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LSEL; L-selectin; Ly22; Ly-22; LYAM1; Lyam-1; Ly-m22; Lymph node homing receptor; lymphocyte adhesion molecule 1; lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; pln homing receptor; PLNHR; SELE; selectin E (endothelial adhesion molecule 1); selectin L; selectin L (lymphocyte adhesion molecule 1); selectin, lymphocyte; Sell; TQ1
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1